WebAug 9, 2010 · Quantity Value Units Method Reference Comment; Δ c H° liquid-8757.9 ± 7.9: kJ/mol: Ccb: Swain, Silbert, et al., 1964: Corresponding Δ f Hº liquid = -752.7 kJ/mol (simple calculation by NIST; no Washburn corrections) WebButyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades. Butyl …
Selectivity and the Properties of a HIC Medium - Sigma-Aldrich
WebHiTrap Butyl FF are prepacked Butyl Sepharose 4 Fast Flow columns for hydrophobic interaction chromatography (HIC).Chromatography columns separate molecules within … WebDec 11, 2013 · Butyl Sepharose 4 Fast Flow can be used with all. aqueous buffers commonly used in. chromatography. The medium has high chemical. and physical stabilities and withstands high. concentrations of denaturing agents such as. guanidine hydrochloride. It also has high thermal. stability and can be autoclaved at 121°C for. personalized photo keychains for him
Sepharose High Performance: Purification with High Resolution
WebG-Sep™ Butyl Agarose Fast Flow (FF) is a 6% cross-linked agarose with butyl groups for Hydrophobic Interaction Chromatography (HIC).The resin is used to separate biomolecules on the basis of relative hydrophobicity. G-Sep™ Agaroses for HIC are also available with octyl and phenyl … WebThe purification of the enzyme was achieved by ultra-filtration, ion-exchange chromatography on DEAE-52, and hydrophobic interaction chromatography on Hitrap Butyl FF column. After the final step of purification, the enzyme was purified 43-fold with a specific activity of 54.4 U/mg, and fair recovery of 46.6%. WebHiPrep Phenyl FF (high sub) 16/10 HiPrep Phenyl FF (low sub) 16/10 HiPrep Butyl FF 16/10 HiPrep Octyl FF 16/10 Instructions 28-9413-25 AB GE Healthcare HiPrep Buffers and solvent resistance De-gas and filter all solutions through a 0.45 µm filter to increase column life-time. Daily use All commonly used aqueous buffers, pH 3–13 personalized photo kitchen towels