Ecar assay kit
WebApr 26, 2024 · The lactate concentration was measured by using a Lactate Assay kit (BioVision, K607-100, Milpitas, CA, USA). ECAR analysis. The extracellular acidification rate (ECAR) assay was performed with ... WebApr 8, 2024 · A Bradford Protein Assay Kit was used to quantify the amount of protein. A total of 20 µg of protein from each sample was mixed with 5× loading buffer and boiled for 5 min. ... D The ECAR in TPC ...
Ecar assay kit
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WebApr 6, 2024 · The activity of mitochondrial respiratory complex I, II and IV, and ATP contents were measured by using commercial kits (BC0515, BC3235, BC0945, and BC0300, Solarbio, Beijing, China). The ECAR assay was conducted using a Seahorse XF-24 extracellular flux analyzer (Seahorse Bioscience, USA). Cells were seeded at a density … WebFeb 7, 2024 · 3.1 The XF24 Assay: Day 1 3.1.1 Seeding Cells in XF24 Cell Culture Plate (Blue Box). 1. The XF24 cell culture plate is designated with rows A–D and columns 1–6 (see Note 1).Plate 50,000,786-O cells in 100 μL growth media per well (see Note 2), leaving background temperature correction wells (A1, B4, C3, and D6) blank with 100 μL growth …
WebApr 4, 2024 · National Center for Biotechnology Information WebApr 2, 2024 · 2.7.2 TUNEL assay. A TUNEL Kit (Servicebio, G1501) was used based on manufacturer's instructions. First, tissue sections were deparaffinized using protease K. Subsequently, membranes were disrupted, the reaction solution was added and nuclei were stained with DAPI. ... Furthermore, ECAR analysis showed that MK2206 treatment …
WebAug 27, 2024 · Some key considerations in flexible bioenergetics assay design are covered in the first section, followed by additional discussion of three topic areas of general importance: 1) cellular energy and ATP, 2) glycolysis, ECAR, and energy partitioning, and 3) mitochondrial dysfunction.
WebJun 1, 2024 · Glycolysis assay was measured using a glycolysis assay kit (Abcam) at 380 nm excitation and 615 nm emission using a SpectraMax M2 microplate reader. ... (F–G) ECAR was assessed in tumor lysates by a microplate fluorometer at 15 min interval. The glycolysis effect was calculated from the ECAR data at 90 min. Data are means and s.d. …
WebJun 1, 2024 · Glycolysis assay was measured using a glycolysis assay kit (Abcam) at 380 nm excitation and 615 nm emission using a SpectraMax M2 microplate reader. ... ricks opticalWebMay 2, 2024 · Extracellular Acidification Rate (ECAR) Assay. The capacity for glycolysis was assessed using the Seahorse XF cell Mito stress test kit or glycolysis stress test kit from Seahorse Bioscience on a Seahorse Bioscience XF96 extracellular flux analyzer (MA, USA). Cells (1.5 × 104 cells/well) were cultured in microplates XF96 (Seahorse … red steamer espressoWebJun 1, 2024 · Agilent Seahorse XFe96 Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of … rickson ridge ymcaWebXF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time. OCR and ECAR rates are key indicators of mitochondrial respiration and … red steam groupsWebSeahorse Xf Glycolysis Stress Test Kit, supplied by Agilent technologies, used in various techniques. ... ECAR values were measured using a Seahorse X96 Bioanalyzer. ... treatments for 6 h as measured by Seahorse glycolytic stress assay. The corresponding AUC of each treatment is shown. D Isotope tracing of [U-13C]-glucose metabolism in … reds teams sportsWebSep 20, 2024 · In simple terms, the EICAR test file is a computer file that was developed to test the response of antivirus (anti-malware) products. It is not a real computer virus, but it mimics malware, and thus allows for safe … rick sousley songsWebOxygen consumption rate (OCR) and extracellular acidification rates (ECAR) were measured with the Mito Stress assay kit, using the XF-96 Extracellular Flux analyser … rick southard