WebPuromycin is an aminonucleoside antibiotic, derived from Streptomyces alboniger, which acts as a protein synthesis inhibitor. It binds to the target ribosome site A, where it is … WebDec 30, 2024 · The working puromycin concentration for mammalian cell lines ranges from 1-10 µg/ml. Prior to using the puromycin antibiotic, titrate the selection agent to determine the optimal concentration for target cell …
Antibiotic Optimization for Cell Transduction Using a Cytotoxicity …
WebPuromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene. Used in cell biology as selection antibiotic in mammalian cell culture systems Recommended working concentration ranges from 0.2–5.0μg/mL WebOct 31, 2024 · The four heterogeneous-intein 3-split Hygromycin Intres enabled 95–100% triple transgenic selection and the two homogeneous-intein Hygro Intres enabled 74–99% triple transgenic selection in... partners in pregnancy clinic peterborough
Puromycin powder, BioReagent, cell culture mammalian 58-58-2
WebThe recommended working concentration in eukaryotic cell culture is 1-10 μg/mL. Used as a selective agent for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). Recommended for use at a range of 1-10 μg/ml. Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C. Packaging WebPuromycin, Dihydrochloride, CAS 58-58-2, is An aminonucleoside antibiotic that inhibits protein synthesis by blocking the translation step and causes premature release of nascent polypeptide chains. Packaging 25 mg in Glass bottle 100 mg in Plastic ampoule Biochem/physiol Actions Primary Target protein synthesis Warning Toxicity: Harmful (C) WebThe recommended dose as a selection agent in cell cultures is within a range of 1-10 g/ml, although it can be toxic to eukaryotic cells at concentrations as low as 1 g/ml. … partners in prevention nj