WebApr 7, 2024 · The dual-parametric QTR-FRET technique enables the linking of guanine nucleotide exchange factor-induced Eu 3+-GTP association to RAS, monitored at 615 nm, and subsequent Eu 3+-GTP-loaded RAS interaction with RAF-RBD-Alexa680 monitored at 730 nm. Both reactions were monitored in a single-well assay applicable for inhibitor … WebFeb 8, 2024 · The Role of Mg21 Cofactor in the Guanine Nucleotide Exchange and GTP Hydrolysis Reactions of Rho Family GTP-binding Proteins* Received for publication, February 8, 2000, and in revised form, April 3, 2000 ... The protein concentrations were determined by using the BCA assay kit (Pierce), and the effective concentrations of …
GAP Assay GAP Activity Assay Application BellBrook Labs
WebMar 16, 2011 · Previous studies show that intra-endosomal acidification by the V-ATPase results in recruitment of the small GTPase Arf6 and its GDP/GTP exchange factor (GEF) ADP-ribosylation factor nucleotide site opener (ARNO) to the endosomal membrane where Arf6 binds to the c-subunit of the V-ATPase while ARNO binds to the a2-isoform . This … WebThe protein concentrations were determined by using the BCA assay kit ... The observations that GEF facilitates GDP/GTP exchange by acting to displace bound Mg 2+ and to destabilize bound nucleotide and that RhoGAP utilizes the Mg 2+ cofactor to elicit catalytic efficiency and specificity in GTP-hydrolysis provide important insight into the GEF ... clive christian rococo after shave
35S GTP Binding Assays GPCR Activation Assay
WebThe Human KRAS WT/SOS1 binding assay can be run in a 96- or 384-well low volume white plate (20 µL final). As described here, compounds or standards are dispensed directly into the assay plate, the pre-mixed GTP and human Tag1-KRAS WT protein is then added together with the human Tag2-SOS1 protein, followed by the dispensing of the HTRF … WebThe GTPase-Glo™ Assay is a homogeneous, bioluminescent assay that is used to measure the activities of GTPases and their associated proteins (GEFs and GAPs) in vitro. The assay quantitates the amount of GTP remaining after a GTPase reaction. With the GTPase-Glo™ Assay, you can measure intrinsic GTPase activity, GAP-stimulated … clive christian perfumes for men