Phm6 vector
Webb1 nov. 2003 · A mutation in the POU4F3 gene (BRN-3.1, BRN3C) is responsible for DFNA15 (MIM 602459), autosomal-dominant nonsyndromic hearing loss. POU4F3 is a member of the POU family of transcription factors and is essential for inner-ear hair cell maintenance. Webb15 juni 2003 · Oligonucleotides carrying Bsu15I and Psp5II restriction sites were introduced in SacII-NotI sites of pHM6 vector. The cDNA encoding the N-terminal part of PTPL1 was cloned by PCR using pSV7d-PTPL1 (gift from C. H. Heldin, Ludwig Institute for Cancer Research, Uppsala, Sweden) as a template in Sac II- Bsu 15I sites of modified pHM6 …
Phm6 vector
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WebbImmunoprecipitation assay of lysates from Neuro-2a cells transfected with pHM6 vector containing HA-tagged p190RhoGEF-C protein or from Neuro-2a cell transfected with … WebbBasic Vector Information; Vector Name: pHM6: Antibiotic Resistance: Ampicillin Length: 5450 bp: Type: Mammalian Expression Vectors Selection Marker: …
Webb15 apr. 2010 · The PCR amplified merB::flag fragment (0.8 kb) was subcloned into the binary expression vector pHM6, downstream of the E12 promoter, the Cauliflower mosaic virus 35 S promoter with two enhancers in tandem, and an omega sequence, to create the plasmid pBMK01. http://www.protocol-online.org/biology-forums/posts/5295.html
Webb1 aug. 2008 · The HA-tagged protein was obtained from a pHM6 vector encoding no protein (Mock). HA-tagged Identification of the domains responsible for interactions between Freud-1/Aki1 and PDK1. (A) The... Webb16 sep. 2009 · The expression plasmid containing the M gene (pHM6-m) or the NP gene (pHM6-np) was then constructed by inserting the M or NP gene into the pHM6 eukaryote …
Webb1 apr. 2015 · Opossum kidney (OK) cells were transfected with pHM6 empty vector (control) or a pHM6-DNPEP construct (DNPEP) and harvested 48 h later. Measurements of monomeric (G) and total cellular actin was performed by DNase I inhibition assay to determine the G/F-actin ratios, the ratio of F-actin content to total cellular protein, and …
WebbPhm6 Expression Vector, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … increased erythropoietin clearance in cancerWebbThe HA-AS160(1–532) construct was obtained by PCR amplification with primers containing KpnI and EcoRI restriction sites and cloned into the pHM6 vector (Roche Applied Science). increased ethanol levelWebbExpression Plasmid Vector Phm6, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … increased estate tax exemptionWebbpHM6 (Search Vector Database) Backbone size w/o insert (bp) 5441 Vector type Mammalian Expression Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 … increased esrWebb16 feb. 2016 · d pHM6 containing celB gene for cloning in rrn16 and 3′rps12 homologous recombination site into chloroplast genome; these vectors contain aadA as selection … increased enzymesWebb17 juni 2005 · Human wild-type ILK cDNA (WT-ILK) in a pFLAG-CMV-2 vector and a pHM6 vector were established in our laboratory. Several ILK fragments that encompassed residues 1–406 (ΔC407- ILK ) and 1–375 (ΔC376- ILK ) were generated by converting appropriate codons in WT- ILK cDNA to stop codons using a QuickChange site-directed … increased eosinophils absWebb16 juni 2014 · OK cells were transiently transfected with pHM6-DNPEP, pHM6-(H94F) DNPEP, or pHM6 empty vector (control) using Lipofectamine 2000 (Invitrogen). Forty-eight hours posttransfection, confluent OK cell monolayers were washed three times in ice-cold PBS, and then biotinylated with EZ-Link NHS-SS- Biotin (Pierce) at 4°C with gentle agitation. increased esr meaning